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"There is a lot of hype about Wembley at the minute and everybody wants to get there," McGuire said.
"All the teams left in it will be itching to be the first to play there so it adds a bit more spice to the game.
"After this round, there will probably be only Super League teams left in it so it's going to be very tough.
"This is the best tie of the round, so it's time for people to step up and I'm sure plenty of players in our team will do that."
Peacock suffered an ankle injury in the win over Catalans, Diskin (groin) and Bailey (calf).
Ali Lauitiiti is also expected to return after picking up a knee injury in Cardiff.
Brian Noble has meanwhile named the same 20 in Wigan Warriors' squad, that went down to St Helens in Cardiff. The Warriors have a proud record in this competition and have inflicted two successive 20-18 defeats to the Rhinos at Headingley.
Michael Withers again misses out through injury, but could be fit for the next Super League encounter against Hull Kingston Rovers.
Former Leeds stars Mark Calderwood and Shane Millard make their returns meanwhile.
Leeds Rhinos 20 man squad: Brent Webb, Scott Donald, Clinton Toopi,Keith Senior, Lee Smith, Danny McGuire, Rob Burrow, Kylie Leuluai, Matt Diskin, Jamie Peacock,Jamie Jones-Buchanan, Gareth Ellis,Kevin Sinfield (C),Ali Lauitiiti,Jamie Thackray,Ryan Bailey, Ian Kirke,Carl Ablett,Jordan Tansey, Ryan Hall.
Wigan Warriors 20 man squad: Chris Ashton, Mark Calderwood, Phil Bailey, Pat Richards, Trent Barrett, Thomas Leuluai, Stuart Fielden, Mickey Higham, Iafeta Paleaaesina, Gareth Hock, Bryan Fletcher, Sean O'Loughlin, David Vaealiki, Shane Millard, Paul Prescott, Harrison Hansen, Darrell Goulding, Joel Tomkins, Liam Colbon, Eamon O'Carroll.