Segeyaro played for the Northern Pride against the Ipswich Jets in the Intrust Super Cup on Saturday afternoon after being left out of the team which defeated Cronulla 30-12 at Toyota Stadium.
The Cowboys will be chasing a fourth straight home win, and fourth consecutive victory overall, when they tackle Manly, who have also won five from seven this season, in what shapes as one of the best games of the round.
North Queensland Toyota Cowboys: 1 Matthew Bowen, 2 Ashley Graham, 3 Antonio Winterstein, 4 Willie Tonga, 5 Kalifa Faifai Loa, 6 Ray Thompson, 7 Johnathan Thurston, 8 Matthew Scott, 9 Aaron Payne, 10 James Tamou, 11 Gavin Cooper, 12 Glenn Hall, 13 Dallas Johnson
Interchange: 14 Scott Bolton, 15 Leeson Ah Mau, 16 Tariq Sims, 17 Ashton Sims, 18 James Segeyaro (one to be omitted).