Tupou replaces Reni Maitua, who has been ruled out of the series with an ankle injury. It is the only change to the side which beat New Zealand 30-18 in a spiteful series opener last weekend.
Scans have revealed a distal syndesmosis injury in Maitua's right ankle, ruling him out of all remaining games in the series against New Zealand and Great Britain. Uncapped Melbourne Storm forward Antonio Kaufusi has been called into the Kangaroos squad for the remainder of the Tri Nations, replacing Maitua.
But Willie Mason has been cleared of injury and will be available for selection for the Test at Telstra Dome.
Australia: Karmichael Hunt, Matt King, Mark Gasnier, Justin Hodges, Greg Inglis, Darren Lockyer (c), Johnathan Thurston, Willie Mason, Cameron Smith, Petero Civoniceva, Andrew Ryan, Nathan Hindmarsh, Luke O'Donnell Interchange: Mark O'Meley, Shaun Berrigan, Anthony Tupou, Brent Kite.
Across the ditch, New Zealand coach Brian McClennan has made two changes to his team for Saturday's Tri-Nations rugby league test against Australia at Melbourne's Telstra Dome.
New Zealand: Brent Webb, Shontayne Hape, Iosia Soliola, Steve Matai, Manu Vatuvei, Nigel Vagana, Stacey Jones, Ruben Wiki (c), Dene Halatau, Roy Asotasi, David Kidwell, Tony Puletua, Simon Mannering Interchange: Jerome Ropati, Nathan Cayless, Adam Blair, David Fa'alogo.Like