The NSW Blues have been named for the first game of the 2017 #Origin series.
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Harlequins finish their campaign with a home fixture against play-off bound Salford on Saturday. A 31-30 victory over Wigan last week secured Quins' Super League status for another year.
They have already begun planning for next season with the signing of Julien Rinaldi from Les Catalans and further new faces are expected to be announced in the near future.
Thomas Leuluai - who has been linked with a move to Wigan - is not included in coach Brian McDermott's 20-man squad. Lee Hopkins (knee) also misses the final match of the season through injury.
Harlequins loose forward Rob Purdham will make his 100th appearance for the London club this weekend. The club's most experienced player Danny Williams plays his last game, as does Nick Bradley-Qalilawa, Neil Budworth, Luke Dorn and Mark Tookey.
The match at the Twickenham Stoop will give Luke Dorn a chance to get to know his future team-mates as he is leaving Quins to join Salford next year.
Salford travel to the capital almost certain of finishing in fifth spot at the end of the regular season. Although they are level with Warrington on 26 points, the City Reds have a better points difference.
Junior Langi is missing from the squad. He has been ruled out for the rest of the season after undergoing surgery on his shoulder.
Harlequins RL v Salford City Reds, Saturday 16th September, kick-off 15:00
Harlequins RL 18-man squad: Bradley-Qalilawa, Budworth, Dorn, Gafa, Haumono, Heckenberg, Lucky Luisi, McLinden, Mills, Noone, Purdham, Randall, Smith, Sykes, Tookey, Wells, Williams, Worrincy.
Salford City Reds 18-man squad: Hodgson, McGuinness, Moule, Dunemann, Robinson, Coley, Alker, Rutgerson, Finnigan, Charles, Highton, Wilshere, Haggerty, Littler, Adamson, Lima, Turner, Brocklehurst.
Referee: S Ganson
Sources: www.skysports.com, www.bbc.co.uk, www.superleague.co.uk